Recombinant enterokinase at 4 ° C, 25 ° C, 37 ° C temperature of the enzyme digestion effect of the control experiment - Database & Sql Blog Articles

Control experiment of recombinant enterokinase digestion at 4 ° C, 25 ° C, 37 ° C temperature

Recombinant enterokinase test batch: REK160401.
The substrate was digested according to the following method, and the electrophoresis was confirmed after a certain time.
0.1 U recombinant enterokinase was digested with 50 ug of substrate for detection.
Time / h 8 16 24 40 48 64 Temperature 4-8 ° C and 25 ° C respectively, the above time time / h 2 4 6 8 24 temperature 37 ° C enzyme cut time
The 4°C digestion effect is as follows:

RESULTS: The substrate was digested at 4 °C, but the digestion time was extended to 48-64 h.


The 25°C digestion results are as follows:

RESULTS: The digestion was carried out at 25 °C for 16 h, but it was ideal for prolonged to 24 h. Even without extension to 64 h, no non-specific digestion occurred.


The 37°C digestion results are as follows:


RESULTS: At 37 °C, the enzyme was completely digested for 8 h, but the substrate also degraded, but the substrate control group did not degrade, indicating that EK had non-specific digestion after 8 h.