Spectrophotometer introduction - Huaqiang Electronic Network

A spectrophotometer is an instrument that quantitatively and qualitatively analyzes a substance by spectrophotometry. The spectrophotometric method is to qualitatively and quantitatively analyze the substance by measuring the absorbance of light at a specific wavelength or a certain wavelength range. With the continuous development of the photometer industry, more and more enterprises and industries have applied photometers, and a large number of enterprises have entered the photometer and developed.
The wavelength of selective absorption of light by a substance, and the corresponding absorption coefficient, is the physical constant of the substance. When the absorption coefficient of a pure substance under certain conditions is known, the test product can be formulated into a solution under the same conditions, and the absorbance can be determined, and the content of the substance in the test sample can be calculated from the above formula. In the visible light region, in addition to some substances that absorb light, many substances are not absorbed by themselves, but they can be added to the color developing reagent under certain conditions or processed to make color and then measured, so it is also called colorimetric analysis. Since there are many factors affecting the color depth when color development, and the instrument with poor purity of monochromatic light is often used, the standard or reference material is used for simultaneous measurement.
Spectrophotometers have become routine instruments in modern molecular biology laboratories. Often used for nucleic acid, protein quantification and quantification of bacterial growth concentrations. The instrument is mainly composed of a light source, a monochromator, a sample chamber, a detector, a signal processor, and a display and storage system.