Tannin detection - Huaqiang Electronic Network

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Tannin test

Keywords: imported reference materials, food testing, pesticide residue testing equipment, food microbiological testing, steel standard materials, multifunctional food safety detector, v-1300, visible spectrophotometer

$Width="678" height="471" src="http://i.bosscdn.com/blog/nophoto.gif" /></p> Abstract: Tannin has strong reducibility and can be Fe3+ Restore to Fe shake. The orange-red complex was formed by the reaction of o-phenanthroline with Fe2+, and the absorbance was measured by a spectrophotometer at r=510 nm and the tannin was determined by comparison with the standard. The content of tannin in fruits was determined by phenanthroline spectrophotometry. 1. Principle Tannin has strong reducibility and can reduce Fe3+ to Fe. The orange-red complex was formed by the reaction of o-phenanthroline with Fe2+, and the absorbance was measured by a spectrophotometer at r=510 nm and the tannin was determined by comparison with the standard. 2. Instrumentation (China) Instrument Co., Ltd. v-1300 visible spectrophotometer. 3. Reagent 1 Tannin (G.R) standard solution, 100ugâ€¢mL-1, diluted 10 times when used. 2FeCl3 (A.R) standard solution, 0.100 molâ€¢L-1. 3 phenanthroline (A.R) solution, 15%. 4NaAc (A.R) solution, O. 1 molâ€¢L-1.4. Pretreatment of samples Samples are grapes, bananas, apples; peach leaves. The fruit to be tested is washed and peeled and chopped. Weigh 15 to 20 g (accurate to 0.1 mg) of the evenly chopped sample and dissolve it quantitatively, and transfer 250 mL quantitatively. In the volumetric flask, shake well, dip it, and dilute with distilled water. Shake well, filter, and the filtrate is the liquid to be tested. 5. Method: Add 0.0, 1.0, 2.0, 3.0, 4.0, 5.0 mL tannin standard solution to 6 50 mL volumetric flasks, and in another 50 mL volumetric flask. Add 5.0 mL of test solution and add 20 mI each. Distilled water, 1.0 mI. FeCI3 solution, 4.0 mL of phenanthroline and 2.0 mL of NaAc solution, dilute to the mark with distilled water, shake well, and measure the absorbance at 510 mm with a 1 cm cuvette after 20 min. The standard amount of the tannin standard solution is taken as the abscissa and the absorbance is plotted on the ordinate. According to the absorbance corresponding to the tannin solution, the tannin content is obtained. 6. Calculate X=C/mKÃ—10-6Ã—100 where: mass fraction of tannin in X-sample, %; content of tannin in sample for C-colorimetry, ug; mâ€”sample mass, g; Kâ€” - the fraction of the colorimetric sample in the sample so called, in this measurement, K = 5 / 250 = 1 / 50. 7. Note Samples should be processed as soon as possible to avoid errors caused by oxidation of tannins. Keywords: imported reference materials, food testing, pesticide residue testing equipment, food microbiological testing, steel standard materials, multifunctional food safety detector, v-1300, visible spectrophotometer</p> </div> </div> <div class="tech- Detail-share">  <div class="bdsharebuttonbox"> <a href="#" class="bds_qzone" data-cmd="qzone" title="Share to QQ Space" ></a> <a href="#" class="bds_tsina" data-cmd="tsina" title="Share to Sina Weibo"></a> <a href="#" class="bds_weixin" Data-cmd="weixin" title="Share to WeChat"></a> <span>Share to:</span> </div> <script>window._bd_share_config = { "common": { "bdSnsKey": {}, "bdText": "", "bdMini": "1", "bdMiniList": false, "bdPic": "", "bdStyle": "2", "bdSize": "16" }, "share ": {} }; with (document) 0[(getElementsByTag Name($

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