Why there is inconsistency in ELISA results - Database & Sql Blog Articles

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According to the summary of annual sales experience, ELISA experiment novices often feedback the ELISA test results of our sales department. The results of different methods in the same laboratory (such as manual and instrument) are inconsistent. This may also be a problem often encountered in every immune laboratory. In this regard, the sales department feedback to the technical department of our company, asking the technology to detail the reasons for the problem according to the actual situation reflected by the customer for the majority of scientific research users to refer to.


There are two main reasons for the inconsistency between different methods and different laboratory results: 1 sensitivity and specificity differences of different methods; 2 different reagents used in different laboratories; 3 different CUTOFF; 4 inter-room quality evaluation.


The main influencing factors of ELISA were: 1 the quality of the specimen; 2 the effect of different time before the addition of the enzyme conjugate on the competition method; 3 the influence of different methods on the test results; 4 incubation; 5 wash plate; 6CUTOFF value.


Solution

1 Do three checks and three pairs to ensure the specimen is correct;

2 "Zhang Guan Li Dai" solution: formulate rules, quality supervision, strict review, fixed personnel, appropriate work intensity;

3 Centrifugation at 3000r/min for 10min;

4 non-anticoagulant;

5 to ensure that the serum is clear, no turbidity.

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